Abstract: In order to acquire hydrologic and acoustic information at the same time,underwater glider is used as synchro observing platform and an algorithm of source localization estimation based on underwater glider in deep water is proposed.Using the hydrologic and acoustic data acquired by a glider in the eastern Indian Ocean,the self-noise of underwater glider and the characteristics of acoustics is analyzed.The underwater track of glider is updated by using underwater dead reckoning.According to the time delay,the underwater sound source localization estimation is realized.The results indicate that the self-noise of underwater glider is quite close to the ambient noise at no-motor time.The sound Transmission Losses(TLs) of experiment and simulation are in great agreement.The underwater sound source localization estimation results are consistent with measurement and the localization error is less than 5%.The underwater glider carrying self-contained hydrophones can get hydrologic and acoustic information at the same time,which is important for the acoustic propagation and target localization.