Abstract: To clarify the mechanism of endolymphatic hydrops caused by Meniere’s disease on vibration characteristics of guinea pig auditory periphery system.The endolymphatic hydrops model was established by injecting the drugs and the vibration characteristics of the healthy and endolymphatic hydrops guinea pig ear sound system were tested by a Doppler laser vibration measurement system.The influences of endolymphatic hydrops on the acoustic characteristics of the guinea pig hearing system were clarified by comparing the results of control group and endolymphatic hydrops group.The results showed that the isolated cochlea of guinea pig had obvious linear characteristics.There was no significant difference in the vibration characteristics of stape and round window membrane between isolated guinea pig cochlea and live guinea pig cochlea.Endolymphatic hydrops reduced the vibration amplitude of the stape and round window membrane,especially in the low frequency and high frequency range.The vibration amplitude of stape decreased from 11.64 nm to 2.27 nm at 500 Hz,and the round window membrane decreased from 24.97 nm to 5.05 nm.At 10 kHz,the round window membrane decreased from 0.45 nm to 0.03 nm.At the same time,endolymphatic hydrops caused a general decrease in the cochlear transmission ratio of guinea pigs,which dropped from 2.82 to 1.46 at 1 kHz,and 2.91 to 0.28 at 10 kHz,respectively.