Abstract: Measurement of mucosal wave velocity on the vocal fold surface is important and useful in investigation of vocal fold tissue mechanics and diagnosis of vocal fold diseases.In this work,Laser Doppler Vibrometer (LDV was used to measure the vibration of the superior surface of the vocal fold,finally reconstructing the shape variation of the superior surface and estimating the mucosal wave velocity through the electroglottography-based time-domain synchronization.In the experiment of excised canine vocal fold vibration,the mucosal wave velocities estimated using LDV were compared equally to those measured by high-speed video.Additionally,the method is able to capture the shape changing of the superior surface in high time resolution.However,because of the limit in single point measurement and time synchronization,the vocal fold vibration should be stable during the measurement to reduce the measurement error.