Abstract: Considering the uncertain effects of temporal and spatial changes in the marine environment on the underwater acoustic environment, we established an ocean-acoustic coupled numerical model and performed a parallel calculation. This model incorporated acoustic calculations into the kinetic ocean, thereby achieving a dynamic forecasting and assessment of the acoustic environment. Furthermore, we adopted the ensemble prediction method to predict the vertical structure of temperature in a classic cross-section, the sound speed of the cross-section of the investigated sea area, and transmission losses. We gave the prediction errors of the sound speed section as well as the 90% probability interval of transmission losses and the uncertainty histograms of the sound speed, transmission losses, and sonar ranges at different depths and frequencies. The results reflected the influence of marine temporal and spacial variations on the uncertainties of the underwater acoustic environment and quantified uncertainties of the underwater acoustic environment. The experimental results indicate that the method used in this study is able to delineate and quantify the uncertainties of the underwater acoustic environment caused by marine dynamic changes.