Abstract: A method based on harmonic salience is proposed for extracting the fundamental frequency from speech signal.It first calculates the harmonic salience spectrum by a inhibiting factor,and summarizes the weighted salience of every harmonic partial.Finally the pitch stream is determined by harmonic tracking.The experiment is conducted with various noised data of TIMIT database and polyphonic melody data of Music Information Retrieval Evaluation Exchange(MIREX) 2005 respectively.The result shows that the accuracy of 88.5%and 73.3%are achieved,and 80%of the half-frequency errors and multi-frequency errors are eliminated.It indicates that this method can effectively enhance the noise immunity and suppress the half-frequency errors and multi-frequency errors.