Abstract: A multi-channel acoustic holographic measurement and analysis system has been developed and been applied to validate sound field separation technique with double holographic planes. Moreover, some methods to enhance separation precision have also been given, based on the error analysis of the technique. The holographic pressures on double planes are measured and analyzed by the developed holographic system when some background sources exist. The experimental results show that the separation technique proposed can suppress the influence of the background sources on holographic reconstruction effectively, and the reconstructed sound field and source identification can be obtained correctly.