Abstract: The mechanism of the performance degration for space-frequency spectrum estimation due to ChannelMismatching and Sensor Coupling is analyzed.To overcome existed methods'limitations that can only compensate oneor two influent factors,a new comprehensive calibration method that can compensates Channel Mismatching and SensorCoupling is proposed.This method improves the calibration precision greatly It is applied to estimate directions andfrequencies simultaneously,and an importallt conclusion that these influent factors can not affect frequency estimationis drawn.This method is easy to be implemented and the results of acoustic experiments in anechoic room show thatthe method is effective.