Abstract: This paper suggested and preliminary studied the sonofluorescent method. The sonofluorescence spectra of three luminol-hydroxide aqueous solutions have been detected and compared with their photofluorescence spectra. The results demonstrated that the fluorescence of the substances can be obtained by sonoexcitation and the fluorescence emission wavelengths,whether the sonoexcitation or the photoexcitation is adopted, are almost in the range of 370-750 um, the emission wavelengths of photofluorescence of luminol aqueous solution, and belong in the visible and near ultraviolet region. Also it seems that, with respect to the photofluorescence of the luminol-hydroxide aqueous solutions, the red shift of the maximum fluorescence peak of the solutions appeared in the sonoexcitation.