Abstract: A psychoacoustic experiment is conducted to investigate the effect of three common sound stimuli presentation methods on echo threshold (ET) of precedence effect. The ETs of four types of stimulus (noise burst, speech, music, pink noise) under three stimuli conditions (Baseline, Buildup, Breakdown) are measured. The results show that the ETs of headphone-based virtual auditory space (VAS) presentation method and loudspeaker-based presentation method have no significant difference, while ETs of them are all significantly different from those of the interaural time difference (ITD) method, especially for long-duration stimuli. The possible reason is the differences in localization cues between various stimulus presentation methods. In addition, with the change of stimuli conditions (from Baseline, Buildup to Breakdown), the dynamic changes of ETs under three stimuli presentation methods are the same, that is, the ETs of the noise burst increase first and then decrease, and the ETs of the three long-duration stimuli do not show significant changes.