Abstract: In order to study the acoustic tweezers with standing wave field and assess the stability on particle manipulation, a phase-modulating model for focused sound field was built using finite element method and the experimental system for acoustic tweezers was constructed. The accuracy of manipulation in multiple directions is measured, and the distribution of the focused sound field and its influence on manipulation were analyzed. The theoretical and experimental results demonstrated that the acoustic tweezers designed is capable of manipulating particles in a plane. Furthermore, it is observed that phase-controlled acoustic tweezers show regional differences in manipulation stability. It is more stable when the manipulation is conducted around the center and less stable towards boundary. Refining the step size during manipulation can enhance stability to some extent.