Abstract: This study investigates the relationship between soundscape sequence elements and emotional regulation, offering guidance for improving public well-being and spatial design. By controlling both the sequence order of sound scenes and key compositional elements, namely sound source, duration, and quantity, the study compares emotional responses elicited before and after sequence design interventions. The results indicate that the redesigned sequences significantly improved emotional experiences, particularly in commercial and transportation spaces, where both pleasantness and arousal ratings increased markedly (p<0.001), accompanied by smoother emotional fluctuation curves. The influence of individual soundscape elements on emotion varied: modifying a single element produced significant but inconsistent effects (p<0.001), while modifying two elements led to consistently significant enhancements in arousal (p<0.001), with the impact on pleasantness depending on the spatial context. Finally, the “soundscape–emotion” regulation model was developed based on confidence ellipse analysis of emotional response data, identifying the directional tendencies and distribution patterns of emotional modulation across different urban space types. The findings provide empirical support for designing emotionally responsive urban soundscapes that promote psychological well-being.